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My ana was low positive at 1:40 speckled, 4 years ago and they did not diagnose me with lupus even though I was suffering with aching muscles, joints, had pleurisy once, got finger infections and had what the doctor thought was rosacea on my face with a negative lupus band test. Now I have been diagnosed with lupus (last week) with the same lab result on my ana (1:40)but this time I had the butterfly rash on my face in the form of hyperpigmentation and of course all the aching joints etc. Does that make sense? Is ana positive at 1:40 or is it considered negative as I have also heard? Also can I have lupus even when I had a negative lupus band test 4 years ago? Leanne